Long committed to the innovation of precise biomedical detection technologies and the development of advanced scientific instruments and innovative medical devices.

The research team led by Huang Guoliang is dedicated to leveraging advanced technical means such as microfluidic chips, biosensors, optical measurement, artificial intelligence (AI), and big data. It aims to maximize the acquisition of precise specific genetic and protein information, as well as structural and functional change information, at multiple levels including molecular, cellular, tissue, organ, small animal, and in vivo human levels, and to visualize such information for multi-modal joint application. Meanwhile, by deeply mining the laws of physiological and pathological characteristics and big data on prior knowledge of renowned medical experts, the team establishes the correlation mechanism between Western medical detection information (molecular, histocytological, imaging, physiological, and biochemical data) and traditional Chinese medicine (TCM) diagnostic indicators (facial appearance, tongue coating, eye signs, pulse condition, and palmistry). This provides advanced detection methods and innovative scientific/medical instrument tools for human health monitoring, early detection, and predictive warning of related diseases.

In the field of public health and epidemic prevention

The team actively participated in the fight against COVID-19. It took charge of developing the Isothermal Amplification Nucleic Acid Analyzer (RTisochipTM-W) and the Isothermal Amplification Microfluidic Chip Nucleic Acid Analyzer (RTisochipTM-A), and participated in the development of microfluidic chip kits for 6 common respiratory viruses including COVID-19. Through the CFDA green channel for COVID-19 prevention and control, all products obtained Class Ⅲ Medical Device Registration Certificates from CFDA on February 22, 2020. With a single sample addition, the devices can complete rapid screening and precise medical molecular diagnosis of 6 common respiratory viruses (including COVID-19) via isothermal nucleic acid amplification within 45 minutes, and have been directly applied in clinical COVID-19 prevention and control. In addition, the team actively participated in the fight against SARS in 2003. In March 2003, it developed the SARS Virus Rapid Detection Microarray Chip Scanner. Together with supporting SARS detection chip kits, the scanner completed testing and verification of over 2,000 clinical samples in more than 40 hospitals in Beijing during the SARS outbreak. The blind test results were directly submitted to the Ministry of Health, which recognized it as the most accurate rapid SARS detection method, playing an important role in SARS prevention and control.

In the field of biochip detection

The team invented innovative technologies such as high numerical aperture (NA) long working distance confocal optical measurement, cylindrical shaped dark-field illumination imaging, 10-million-level resolution digital rapid thickness measurement, and medium-pore reflective high-efficiency light splitting. It developed more than 10 types of key components: for example, high-quality objectives with NA=0.72 and working distance >3mm (conventional objectives have NA=0.6 and working distance <1mm); medium-pore reflective light splitting structures with a combined transmission-reflection efficiency of >97% across the entire visible spectrum (conventional broadband beam splitters have <25% combined efficiency, and narrowband dichroic mirrors have 70%). The team completed the development and industrialization of 4 types of scientific/medical instruments, solving major microscopic technical bottlenecks such as the trade-off between numerical aperture and working distance, light splitting efficiency and wavelength range, and resolution and imaging area. This achieved fluorescence detection efficiency reaching the optical diffraction limit of system design, with the instrument cost being less than 1/10 of imported equipment. The technical appraisal organized by the Ministry of Education concluded that "the optical design and instrument performance are at the international leading level, filling the gap in domestic biochip detection instruments." Currently, the products occupy 70% of the domestic biochip detection instrument market, with sales revenue exceeding 250 million yuan. For the first time, domestic biochip detection instruments were exported to European and American markets, serving over 300 users and earning more than 6 million US dollars in foreign exchange. This provides platform instrument support for China's biochip industry to lead the world and promote its application.

In the field of micro-nano biomedical detection

The team invented isothermal amplification molecular diagnosis technologies and systems for micro-nano systems, including innovative technologies such as microfluidic chip-based isothermal amplification, superhydrophobic inert treatment, centrifugal sample injection air-isolated microfluidic chips, dual-focal-plane spatial filtering optical detection structures, and sub-millimeter air bath heating and temperature control. These solved multiple bottleneck technologies in low-cost precision medicine. The team completed the development and industrialization of 5 types of scientific/medical instruments, with sample reagent consumption of 0.94 μL, sensitivity of 10 copies, and results available in 45 minutes (conventional PCR requires 25 μL, 1000 copies, and 120 minutes). The technical appraisal organized by the Ministry of Education concluded that "the project technology is at the international leading level, filling the gap in domestic micro-nano biomedical detection scientific instruments and medical devices." The products have obtained 1 National Innovative Medical Device Approval from CFDA, 2 Class Ⅲ Medical Device Registration Certificates, and 1 CE Certificate. They have been demonstrated and applied in more than 400 hospitals in China and obtained medical device registration certificates in Thailand, setting an example for exports to Belt and Road countries. Since the instrument was launched in 2015, combined with supporting kits, it has achieved sales revenue exceeding 500 million yuan, laying a platform technical foundation for personalized low-cost precision medicine.

In the field of multi-level in situ measurement covering molecular, cellular, organ, small animal, and human levels

The team invented innovative technologies such as white light interferometric hyperspectral super-resolution imaging, steam bath dark-phase contrast gene point mutation naked-eye visualization, in situ detection of in vitro fertilization of chip oocytes, AI-based precise analysis of TCM eye signs for health assessment with shadow-free sclera imaging, optical-X-ray dual-mode coaxial in vivo imaging with a single detector, multi-level microstructured flexible sensing, and cuffless continuous blood pressure measurement. Among them, the AI-based TCM eye sign health analysis instrument has been demonstrated and applied in more than 200 units in China, achieving direct economic benefits exceeding 100 million yuan. These technologies solved multiple common bottleneck problems: for example, optical measurement is limited by diffraction while super-resolution detection relies on fluorescent labeling; assisted reproductive manipulation is difficult and lacks evaluation methods; X-ray and optical dual-mode imaging requires two different detectors in separate spaces; wide-field imaging of the eyeball is severely interfered by reflected light while slit lamps can only observe narrow-band areas. The technologies realize multi-level, multi-modal in situ measurement from molecular to human levels, and will provide innovative multi-level measurement methods and advanced instruments covering molecular to human levels for life sciences, medicine, clinical practice, and precise TCM.

1.Anni Deng, Zeyin Mao, Xiangyu Jin,Wenqi Lv, Leyang Huang, Hao Zhong, Shihong Wang, Yixuan Shi, Qin Huang, Jianxin Zhao, Li Ma, Xianbo Luo, Tianqi Zhou, Heng Zou, Rongxin Fu and Guoliang Huang*（通讯作者），ID-CRISPR: A CRISPR/Cas12a Platform for Label-Free and Sensitive Detection of Rare Mutant Alleles Using Self-Interference DNA Hydrogel Reporter，Biosensors and Bioelectronics，2025，278:117309（1-8）.

2.Zeyin Mao, Anni Deng, Xiangyu Jin, Tianqi Zhou, Shuailong Zhang, Meng Li, Wenqi Lv, Leyang Huang, Hao Zhong, Shihong Wang, Yixuan Shi, Lei Zhang*, Qinping Liao*, Rongxin Fu*, and Guoliang Huang*（通讯作者），Highly-specific and rapid multiplex identification of Candida species using digital microfluidics integrated with semi-nested genoarray，ANALYTICAL CHEMISTRY, 2024, https://doi.org/10.1021/acs.analchem.4c04265.

3.Han Yang, Rongxin Fu, Xiaohui Shan, Xue Lin, Ya Su, Xiangyu Jin, Wenli Du, Wenqi Lv, Guoliang Huang*（通讯作者）, A nature-inspired hierarchical branching structure pressure sensor with high sensitivity and wide dynamic range for versatile medical wearables, Biosensors and Bioelectronics，2022，203:114028.

4.Wenqi Lv, Ying Song, Rongxin Fu, Xue Lin, Ya Su, Xiangyu Jin , Han Yang, Xiaohui Shan, Wenli Du, Qin Huang, Hao Zhong, Kai Jiang, Zhi Zhang*, Lina Wang* and Guoliang Huang*（通讯作者） , Deep Learning Algorithm for Automated Detection of Polycystic Ovary Syndrome Using Scleral Images , Frontiers in Endocrinology,2022, 12:789878(1-8)

5.Rongxin Fu, Wenli Du, Xiangyu Jin, Ruliang Wang, Xue Lin, Ya Su, Han Yang, Xiaohui Shan, Wenqi Lv,Zhi Zheng,* and Guoliang Huang*（通讯作者）, Microfluidic Biosensor for Rapid Nucleic Acid Quantitation Based on Hyperspectral Interferometric Amplicon-Complex Analysis, ACS Sensors, 2021, DOI:10.1021/acssensors.1c01491.

6.Ya Su, Rongxin Fu, Wenli Du, Han Yang, Li Ma, Xianbo Luo, Ruliang Wang, Xue Lin, Xiangyu Jin,Xiaohui Shan, Wenqi Lv and Guoliang Huang*，Label-Free and Quantitative Dry Mass Monitoring for Single Cells during In Situ Culture，Cells， 2021, 10:1635. https://doi.org/10.3390/cells10071635.

7.Ruliang Wang, Han Yang, Rongxin Fu , Ya Su, Xue Lin, Xiangyu Jin, Wenli Du,Xiaohui Shan and Guoliang Huang*（通讯作者）, Biomimetic Upconversion Nanoparticles and Gold Nanoparticles for Novel Simultaneous Dual-Modal Imaging-Guided Photothermal Therapy of Cancer, Cancers 2020, 12, 3136.

8.Guoliang Huang#,*（第一作者和通讯作者）, Qin Huang#, Lan Xie#, Guangxin Xiang, Lei Wang,Hui Xu, Li Ma, Xianbo Luo, Juan Xin, Xinying Zhou, Xiangyu Jin, and Lei Zhang，A rapid, low-cost, and microfluidic chip-based system for parallel identification of multiple clinical pneumonia-related pathogens，Scientific Reports，7 (1) :6441（1-10）, 2017。

9.Qi Li#，Rongxin Fu#，Junqi Zhang, Ruliang Wang, Jiancheng Ye, Ning Xue, Xue Lin, Ya Su, Wupeng Gan, Ying Lu, and Guoliang Huang*（通讯作者），A label-free method using weighted-phase algorithm to quantitate nanoscale interactions between molecules on DNA microarray，Analytical Chemistry, 89(6)，3501–3507, 2017.

10.Junqi Zhang#, Rongxin Fu#, Liping Xie, Qi Li, Wenhan Zhou, Ruliang Wang, Jiancheng Ye, Dong Wang, Ning Xue, Xue Lin, Ying Lu and Guoliang Huang*（通讯作者），A smart device for label-free and real-time detection of gene point mutations based on the high dark phase contrast of vapor condensation，Lab on a Chip, 15: 3891–3896, 2015.

Academic Honors and Awards

1.2024: Innovation and Application of Key Technologies for Craniocerebral War Trauma Treatment in Special Battlefield Environments, Second-Class National Science and Technology Progress Award, awarded by the Central Committee of the Communist Party of China and the State Council of the People's Republic of China.

2.2019: Isothermal Amplification Microfluidic Chip Nucleic Acid Analysis System and Its Application, Second-Class Science and Technology Progress Award of Institutions of Higher Education, awarded by the Ministry of Education.

3.2017: R&D and Application of Multi-Indicator Microfluidic Chip Detection System for Respiratory Pathogens, Second-Class Beijing Municipal Science and Technology Award, awarded by the People's Government of Beijing Municipality.

4.2016: A Detection System and Method for Micro/Nanoliter Volume Fluid Chips, Gold Award of Beijing Invention and Innovation Competition, awarded by the Beijing Invention Association.

5.2015: Research on Isothermal Amplification Microfluidic Chip Nucleic Acid Analyzer, First-Class Science and Technology Award of the Chinese Instrument and Control Society, awarded by the Chinese Instrument and Control Society.

6.2008: Biochips: The CPU of Life Information, Golden Signature Award (Creation Category) of the 4th Wu Dayou Popular Science Book Award, awarded by the Wu Dayou Popular Science Book Award Selection Committee.

7.2008: Development and Application of Systematic Biochips and Related Instruments/Equipment, Second-Class Beijing Municipal Science and Technology Award, awarded by the People's Government of Beijing Municipality.

8.2007: Development and Application of Systematic Biochips and Related Instruments/Equipment, Second-Class National Technology Invention Award, awarded by the State Council of the People's Republic of China.

9.2005: Development of Microarray Chip Scanner, Second-Class Beijing Municipal Science and Technology Award, awarded by the People's Government of Beijing Municipality.

Courses Offered

1.Biomedical Detection - Optical Instrument Design

Other Social Positions

1.Member of the Medical Instrument Subcommittee of the National Technical Committee for Standardization.

2.Senior Member of the Chinese Optical Society; Vice Chairman of the Biomedical Photonics Branch of the Chinese Society of Biomedical Engineering.

3.Review Expert for the National Natural Science Foundation of China, Key R&D Programs of the Ministry of Science and Technology, and fund projects of Beijing, Shandong and other provinces/municipalities.

4.Editorial Board Member/Reviewer for journals including Analytical Methods, Analytica Chimica Acta, Science China Chemistry, Acta Optica Sinica, Journal of Optoelectronics·Laser, Journal of Biomedical Engineering, Acta Biophysica Sinica, and Journal of Beijing Institute of Technology.

Technical Patents

1.Huang Guoliang, Cheng Jing, Zhou Yuxiang, Feng Jihong, Yang Rong, Liu Chengxun, Li Lin. Lens for Biochip Scanning Detection System. Authorization Date: December 22, 2004. China Invention Patent, Patent No.: ZL01115426.8.

2.Huang Guoliang, Cheng Jing, Wang Xianhua, Song Xiaobo, Chen Renyuan, Xiao Ming, Zhu Jiang, Yang Yang, Dong Zhonghua. Microarray Chip Detection System. Authorization Date: September 10, 2008. China Invention Patent, Patent No.: ZL2005100632089.

3.Huang Guoliang, Bai Jing, Shan Baoci, Dong Hongying. Rotating Platform-Based Multi-Modal In Vivo Imaging Detection System for Small Animals. Authorization Date: January 14, 2009. China Invention Patent, Patent No.: ZL200610144284.7.

4.Huang Guoliang, Yang Xiaoyong, Zhang Rong, Zhou Cheng, Hu Yuming, Li Guanxing, Du Guibin, Huang Mingxian, Cheng Jing. A Detection System and Method for Micro/Nanoliter Volume Fluid Chips. Authorization Date: September 2, 2009. China Invention Patent, Patent No.: ZL200710122151.4.

5.Qiu Tian, Huang Guoliang, Cheng Jing. An Optical Fiber Reflective Spectrophotometer for Micro/Nano Systems and Its Application. Authorization Date: March 27, 2013. China Invention Patent, Patent No.: ZL200910236148.4.

6.Wang Tongzhou, Huang Guoliang, Ma Li, Luo Xianbo, Tian Hao, Huang Jie, Li Xin. Digital Signal Processing Method for Microfluidic Chip Detection and Applied Detection Device. Authorization Date: April 24, 2013. China Invention Patent, Patent No.: ZL201110113608.1.

7.Han Chao, Huang Guoliang, Cheng Jing. An Integrated Microfluidic Chip Device and Its Usage Method. Authorization Date: July 4, 2012. China Invention Patent, Patent No.: ZL201010218520.1; International PCT Patent, Patent No.: PCT/CN2011/001013.

8.Zhang Guohao, Huang Guoliang, Wang Can, Guo Su, Wang Lei, Xing Wanli, Cheng Jing. A Microfluidic Chip and Its Application. Authorization Date: June 11, 2014. China Invention Patent, Patent No.: ZL201210311357.2; International Patent, Patent No.: PCT/CN2013/001004.

9.Li Qiang, Huang Guoliang, Wang Tongzhou, Li Xin, Zhao Songmin. A Microcarrier Biochip and Its Application. Authorization Date: February 24, 2016. China Invention Patent, Patent No.: ZL201210166887.2.

10.Huang Guoliang, Bai Jing, Zhao Songmin, Wang Tongzhou, Li Xin. Multi-Modal In Vivo Imaging Detection System with Resolution of at Least 50μm. Authorization Date: March 5, 2014. China Invention Patent, Patent No.: ZL201210353038.8.

11.Huang Guoliang, Wang Tongzhou, Li Qiang, Li Xin, Zhao Songmin, Ma Li, Luo Xianbo, Pang Biao. Full-Spectrum Super-Resolution Measurement Method and Label-Free Biomolecule Measurement System. Authorization Date: June 3, 2015. China Invention Patent, Patent No.: ZL201310043661.8.

12.Huang Guoliang, Wang Ruliang, Lin Xue, Xue Ning, Bai Jing. Dual-Modal Coaxial In Vivo Imaging Method and System. Authorization Date: February 2, 2018. China Invention Patent, Patent No.: ZL201510165665.2. Issued by the State Intellectual Property Office of the People's Republic of China.

13.Huang Guoliang, Wang Tongzhou, Zhang Junqi, Li Qi, Fu Rongxin, Huang Qin, Wang Ruliang, Huang Shiguang, Zhang Zining. An Interferometric Rotational Mapping Detection Method with Lateral Resolution of 1nm. Authorization Date: August 24, 2016. China Invention Patent, Patent No.: ZL201410277169.1.

14.Huang Guoliang, Lin Xue, Xue Ning, Ma Li, Luo Xianbo, et al. A High-Throughput Microfluidic Chip Nucleic Acid Amplification Analysis and Detection System. Authorization Date: June 29, 2018. China Invention Patent, Patent No.: ZL201610429432.3.

15.Huang Guoliang, Fu Rongxin, Su Ya, Jin Xiangyu, Yang Han. Hyperspectral Interferometric Label-Free Imaging Method and Living Cell Quantitative Tomographic Imaging System. Authorization Date: June 2, 2020. China Invention Patent, Patent No.: ZL201910387744.6.

16.Huang Guoliang, Jin Xiangyu, Fu Rongxin, Shan Xiaohui, Du Wenli. Optical Fiber Sensing Microfluidic Chip Nucleic Acid Amplification In-Situ Real-Time Detection System and Method. China Invention Patent, Patent No.: ZL201911111985.4. Application Date: November 14, 2019; Authorization Date: June 29, 2021.

17.Huang Guoliang, Lü Wenqi, Jiang Kai, Fu Rongxin. An Artificial Intelligence Multi-Modal Imaging Analysis Device. China Invention Patent, Patent No.: ZL201911392016.0. Application Date: December 30, 2019; Authorized. PCT Patent, Patent No.: PCT/CN2020/123568. Application Date: October 26, 2020; Authorization Date: June 28, 2022.

18.Huang Guoliang, Yang Han, Fu Rongxin, Luo Xianbo, Lü Wenqi. Flexible Pressure Sensor Based on Multi-Level Structure, Preparation Method and Measurement System. China Invention Patent, Patent No.: ZL202110196116.7. Application Date: February 22, 2021; Authorization Date: June 28, 2022.

19.Huang Guoliang, Fu Rongxin, Lü Wenqi, Du Wenli, Shan Xiaohui, Jin Xiangyu, Lin Xue. Rapid Detection Method for Sampling Swabs and Fully Integrated Analysis System for Swab Sampling. China Invention Patent, Patent No.: ZL202110829407.5. Application Date: July 22, 2021; Authorization Date: July 26, 2022.

20.Huang Guoliang, Lin Xue, Lü Wenqi, Du Wenli, Shan Xiaohui, Fu Rongxin. Combined Detection System of Non-Invasive Imaging Screening and Minimally Invasive Sampling Nucleic Acid Typing. China Invention Patent, Patent No.: ZL202110829210.1. Application Date: July 22, 2021; Authorization Date: November 22, 2024.